백서의 치주인대세포와 두개관세포의 혼합배양이 석회희과정에 미치는 영향
The effects of mixed culture of rat periodontal ligament cells and calvaria cells on the calcification
김지숙, 박준용, 이만섭, 권영혁, 허익, 임상철,
소속 상세정보
김지숙 ( ) - 경희대학교 치과대학 치주과학교실
박준용 ( ) - 경희대학교 치과대학 치주과학교실
이만섭 ( ) - 경희대학교 치과대학 치주과학교실
권영혁 ( ) - 경희대학교 치과대학 치주과학교실
허익 ( ) - 경희대학교 치과대학 치주과학교실
임상철 ( ) - 경희대학교 치과대학 치주과학교실
KMID : 0363019970270040923
Abstract
This study was performed to evaluate the effect of mixed culture of rat´s calvana cells and periodontal ligament cells on calcification. These cells have been known to do important role on the periodontal tissue regeneration, especially alveolar bone and cementum Experimental groups were made which based on the different rate of rat´s calvaria cells and periodontal ligament cells, and then these cells were cultured with Dulbecco´s Modified Eagle´s Medium contained with 10% fetal bovine serum, 50fg/ml ascorbic acid, and 10mM/ ml Na-aglycerophosphate. Each group was characterized by examining the cell proliferation rate, amount of total protein synthesis, alkaline phosphatase activity, and the number of calcified nodules in vitro. In cell proliferation rate , the cells of control groups were cultured Dulbecco´s Modified Eagle´s Medium contained with 10 % fetal bovine serum.
The results were as follows
1. The cell proliferation rate in control groups decreased stastically significantly along with the decrease of the rate of bone cells at 7 day and 20 day (P < 0.01).
2. The cell proliferation rate in experimental groups decreased stastically significantly along with decrease of the rate of bone cells at 3 day and 14 day (P < 0.01).
3. The amount of total protein synthesis was significantly decreased along with decrease of the rate of bone cells at 3 day and 6 day (P < 0.01).
4. Alkaline phosphatase activity showed reverse time dependent pattern and was significantly decreased along with decrease of the rate of bone cells during the experimental periods (P < 0.01).
5. Calcified nodules were observed in group 1 (Rat calvaria cells alone) for the first time, and the number of calcified nodule decreased stastically significantly along with the decrease of the rate of bone cells at 12 day (P < 0.01).
From the above results, When bone cells and periodontal ligament cells were mixed cultured, the cell proliferation rate was mostly dependent on the actual rate of bone cells and same pattern was showed in amount of total protein synthesis, alkalinephosphatase activity, and the number of calcified nodules. And the calcified nodule forming capacity of bone cells was inhibited by periodontal ligament cells
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